Part:BBa_K2325000

Formamidase (FOR)

The BBa_K2325000 contains a coding sequence of formamidase which catalyzes the conversion of formamide to ammonia.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 69
  Illegal NgoMIV site found at 556
  Illegal NgoMIV site found at 751
  Illegal NgoMIV site found at 898
  
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.

HCO - NH₂ + H₂O ⇒ HCOOH + NH₃

Gel analysis

Enzymatic properties

Figure 1: Enzymatic activity of formamidase under different temperatures and pH

Figure 2: The Michaelis constant of formamidase	Figure 3: Influence of metal ion on the enzymatic activity of formamidase